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Image Search Results
Journal: Journal of sport and health science
Article Title: BMAL1/PGC1α4-FNDC5/irisin axis impacts distinct outcomes of time-of-day resistance exercise.
doi: 10.1016/j.jshs.2024.100968
Figure Lengend Snippet: Fig. 7. Irisin increased muscle hypertrophy via aV class of integrins and FAK-AKT-CREB signaling. Representative western blot analysis and quantification of (A) integrin-like signaling FAK-AKT-CREB phosphorylation and (B) Quantitative Polymerase Chain Reaction (qPCR) analysis in differentiated C2C12 myotubes pre-treated with negative control RGD (Con) or RGDS (10 uM) for 10 min and then treated with recombinant irisin (600 ng/mL) or His-tag (600 ng/mL). Repre- sentative Western blot analysis and quantification of (C) integrin-like signaling FAK-AKT-CREB phosphorylation and (D) qPCR analysis of IL6 in gastrocnemius muscle (GAS) from mice i.p. injected with His-tag or recombinant irisin (1mg/kg) and treated with negative control RGD (Con) or cRGDyK (1mg/kg) peptide every day for 2 weeks. (E) Mice were treated with integrin aV inhibitor cRGDyK (1 mg/kg) or negative control RGD (Con) for 48 h and their grip strength was assessed at late rest (LR) or late active (LA) phase. n = 6 per group. Percentage of (F) body weight change, (G) representative Hematoxylin and Eosin (H&E) staining, and (H) quantification of average fiber sizes of GAS muscles from mice i.p. injected with His-tag or recombinant irisin (1 mg/kg) in the combination of negative control RGD peptide (Con) or cRGDyK (1 mg/kg) every day for 2 weeks. n = 4 per group. Scale bar = 100 um. All data were shown as mean § SEM. Two-tailed unpaired Student’s t test was used for statistical comparison. *p < 0.05; ** p < 0.01. C2C12 = C2 myoblast cell line 12; GAPDH = Glyceraldehyde-3- phosphate Dehydrogenase; ns = not significant; RGDS = Arg-Gly-Asp-Ser.
Article Snippet: For quantitative real-time-polymerase chain reaction (qRT-PCR) and western blot analysis, differentiated C2C12 myotubes were pre-treated with 10 mM negative control Arginyl-glycyl-aspartic acid (RGD) (GL Biochem Chemicals, Shanghai, China) or
Techniques: Western Blot, Phospho-proteomics, Real-time Polymerase Chain Reaction, Negative Control, Recombinant, Injection, Staining, Muscles, Two Tailed Test, Comparison